Dengue virus (DENV) and Yellow fever virus (YFV) are arboviruses transmitted by some species of Aedes mosquitoes. The preponderance of Aedes mosquitoes in Zaria has been reported but the incidence of viruses in the mosquito populations is not known. The study used qualitative real time RT-PCR to investigate the prevalence of DENV and YFV in Aedes populations by targeting the regulatory 3' UTR genome region present in the four Dengue sero-types (DENV-1, -2, -3 and -4) and the NS 5 of YFV. A total of 257 female Aedes from five species; Aedes aegypti, Ae. furcifer, Ae. taylori, Ae. metallicus and Ae. vittatus were identified and pooled. Using total RNA extracted from samples, DENV was detected in 10 Aedes pools (40%) while the NS 5 gene for YFV was detected in 15 Aedes pools (60%). The dominant species with DENV and YFV were Aedes aegypti and Ae. furcifer. Except for Kufena, all locations showed presence of DENV while YFV was present in all locations. The study concludes that the two aboviruses are circulating in the Aedes mosquito, posing a risk to the human population.
Department of Biological Sciences, Ahmadu Bello University, Zaria, Nigeria